[pY185]MAP (177 – 189) kinase

<p>[pY185]MAP (177-189) is a synthetic phosphopeptide substrate designed for kinase assay applications, corresponding to residues 177-189 of the mitogen-activated protein (MAP) kinase family with a phosphorylated tyrosine at position 185 (or its equivalent position within the peptide sequence). </p>

SupplierGlobal Technology Co., Ltd.www.hqtechtirz.com

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TECHNICAL SPECIFICATIONS

Item	Specification
Product Name	[pY185]MAP Kinase (177-189)
CAS NO.	/
Appearance	White to off-white lyophilized powder
Molecular Formula	C67H100N18O22
Molecular Weight	1509.65g/mol
Purity (RP-HPLC)	≥ 95.0%
Solubility	Soluble in water, 0.1% TFA, or DMSO (up to 10 mg/mL)
Endotoxin	≤1.0 EU/mg
Biological Activity	Specifically recognized by anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibodies.
Storage Conditions	Store at -20°C; stable for 12 months when stored desiccated. Avoid repeated freeze-thaw cycles.
Stock Location	Stock in USA
MOQ	1g

1.MECHANISM OF ACTION

This peptide does not have enzymatic activity but functions as a specific molecular tool to study phosphorylation-mediated protein interactions. The phosphorylated tyrosine residue (pY185) within its sequence mimics the key activation loop phosphorylation event in MAP kinases (e.g., pT185/pY187 in ERK2).

2.PRODUCT INDICATIONS

Antibody Validation: Serving as a critical positive control to test the specificity and sensitivity of phospho-specific antibodies directed against the activation loop of MAP kinases (e.g., anti-phospho-ERK1/2).
Protein-Protein Interaction Studies: Used in pull-down assays, surface plasmon resonance (SPR), or microscale thermophoresis (MST) to study the binding affinity and kinetics of SH2 or PTB domain-containing proteins (e.g., Grb2, Shc) to the phosphorylated MAP kinase motif.
Phosphatase Assays: Acting as a defined substrate to measure the activity of protein tyrosine phosphatases (PTPs) or dual-specificity phosphatases (DUSPs) that dephosphorylate and inactivate MAP kinases.
Calibration Standard: Quantifying the amount of phosphorylated MAP kinase in samples when used as a standard in quantitative phosphoproteomic or targeted mass spectrometry assays.

3.CLINICAL EFFICACY OF PRODUCT

Research & Diagnostic Tool Efficacy: Its "efficacy" is defined by its ability to generate reliable, reproducible data in the applications listed above. It is essential for validating reagents used in both research and clinical pathology assays that measure phospho-MAPK as a biomarker.
Biomarker Development Support: Phosphorylated MAP kinases (e.g., p-ERK) are important biomarkers in cancer and inflammatory diseases. This peptide is used to develop and validate diagnostic antibodies and assays that measure these biomarkers in patient tumor samples, influencing prognosis and treatment decisions.

4.DELIVERY & SHIPPING

We specialize in global logistics for various cosmetic peptides. Your order will be handled with the utmost care, ensuring safe, reliable, and intact delivery.
Professional and Secure Packaging
Each bottle of peptide is protected with a special packaging solution to ensure stability and integrity during transportation.
We can also customize packaging according to customer needs.
Shipping Methods
Main Method: International Air Freight

We primarily use DHL, FedEx, UPS, and TNT because of their global reliability, speed, and advanced tracking systems. Air freight ensures the fastest transit time and minimizes the impact of environmental factors on the product.